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A los cebadores forward de cada microsatélite durante la PCR se hibridizó la secuencia universal M13 con fluorocromos (PET, NED, VIC y FAM) en el extremo 5' para la genotipificación (Schuelke, 2000). | During PCR, the forward primers of each microsatellite were hybridized to the universal M13 sequence with fluorochromes (PET, NED, VIC and FAM) at the 5' end for genotyping (Schuelke, 2000). |
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