cdna
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El laser excita la fluorescencia del cDNA, generando señales. | The laser excites fluorescence of the cDNA, generating signals. |
Posteriormente, el RNA obtenido se retrotranscribió a cDNA (QuantiTec Reverse Transcription, Qiagen). | Subsequently, the RNA obtained was retrotranscribed to cDNA (QuantiTec Reverse Transcription, Qiagen). |
El cDNA está conforme a la mutagénesis, que altera ligeramente la estructura resultante de la proteína. | The cDNA is subject to mutagenesis, which slightly alters the resultant protein structure. |
El procedimiento esencialmente simplifica la secuencia conectando los segmentos del cDNA juntos en una cadena larga. | The procedure essentially simplifies sequencing by linking the cDNA segments together in a long chain. |
Se amplifica el cDNA hasta que haya 100.000 copias de la serie en cada moldura. | The cDNA is amplified until there are 100,000 copies of the sequence on each bead. |
Los 5 segmentos completos de cDNA del gen SI fueron amplificados de las 5 cepas aisladas con RT-PCR. | The 5 full-length cDNA segments of the SI gene were amplified from the 5 isolated strains with RT-PCR. |
El ARN de mensajero (mRNA) se aísla de la muestra y se convierte en la DNA elogiosa (el cDNA) | Messenger RNA (mRNA) is isolated from the sample and converted into complimentary DNA (cDNA) |
Se trata de aislar el RNA mensajero que codifica para una proteína concreta y obtener su correspondiente DNA complementario (cDNA). | The messenger RNA encoding a particular protein is isolated and the corresponding complementary DNA (cDNA) obtained. |
El cDNA encuadernado indica un gen se exprese que porque los cabos elogiosos del socio están ya dentro del microarray. | The bound cDNA indicates a gene that is expressed because the complimentary partner strands are already within the microarray. |
Mediante la clonación y secuenciación parcial de cDNA, se identificó finalmente al agente causal como el CDV (Schubert et al., 2006). | By means of cloning and partial cDNA sequencing the causal agent was eventually identified as CDV (Schubert et al., 2006). |
Puede ser utilizada como enzima dentro de un ZMW y en permitir síntesis complementaria directa de la DNA (cDNA) en tiempo real. | It can be used as an enzyme within a ZMW and in allowing direct complementary DNA (cDNA) synthesis in real time. |
Se elaboró una librería subtractiva de cDNA del riñón cefálico estimulado con LPS de mero malabárico utilizando hibridación substractiva por supresión. | A subtracted cDNA library from the head kidney of LPS-stimulated Malabar grouper was constructed using suppression subtractive hybridization. |
El diseño y el uso de un microserie de cDNA salmón Atlántico hecho de encargo, estudiar los interacciones de la reciba y patógenoen en salmonids. | Design and use of a custom Atlantic salmon cDNA microarray for study of host-pathogen interactions in salmonids. |
Pueden ser utilizados en el punto-de-cuidado y son generados observando el cDNA en las diapositivas de cristal que entonces se traducen para apuntar las proteínas. | They can be used at point-of-care and are generated by spotting cDNA on glass slides which are then translated to target proteins. |
Pues cada sitio de la muestra espera un diverso gen, el cDNA atará solamente a los sitios que esperan genes específicos con pares bajos elogiosos. | As each sample spot holds a different gene, the cDNA will only bind to spots that hold specific genes with complimentary base pairs. |
Macrogen Inc. Ofrece varios servicios para el análisis de Genoma a partir de distintos enfoques como el de Shotgun, cDNA, BAC, Cosmid, y Fosmid. | Macrogen Inc. provides various services to analyze genome by using various approaches such as Shotgun, cDNA, BAC, Cosmid, and Fosmid. |
Los cDNA amplificados a partir de KLF4, SOX2 y c-MYC-T58A se clonaron en un vector modificado pMSCV puro que permite la expresión de N-terminal marcado con Proteina FLAG. | The amplified cDNAs from KLF4, SOX2 and c-MYC-T58A were cloned into a modified pMSCVpuro vector that allows the expression of N-terminal FLAG-tagged proteins. |
El cDNA se diluyó a una razón de 1/5 y se utilizó 0,5 µl de muestra por pocillo, siguiendo el protocolo descrito por el proveedor. | The cDNA was diluted at a ratio of 1/5 and 0.5 µl of sample per well was used, following the protocol described by the supplier. |
A partir de 2 µg de ARN total, se obtuvo el ADNc utilizando el kit High Capacity cDNA Reverse Transcription (Applied Biosystems) siguiendo las instrucciones del fabricante. | From 2 µg of total RNA, the cDNA was obtained using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems) following the manufacturer's instructions. |
Para la síntesis de cDNA se uso 1 μg de ARN y se siguieron las instrucciones del fabricante para el kit Superscript III First Strand (Invitrogen). | For the synthesis of cDNA 1 µg of RNA was used and the instructions of the maker of the Superscript III First Strand kit (Invitrogen) were followed. |
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