What is wrong with you?
Is it "que es tu problema?"
Welcome to the forum!
Sure, that would be a possibility. "¿Qué te pasa?..¿Qué te ocurre?..¿Qué te sucede?" are all possibilities also.
Just as we can say different things, such as "What's wrong with you/What's the problem/Do you have a ;problem?" in English, there are many possibilities in Spanish.
FYI. ¿Qué te pasa? = I wonder what is wrong with you, and I am possibly worried about you.....What's going on with you?
¿Cuál es tu problema? = You have a problem, possibly with me, and I would probably like to fight about it, so let's get angry and yell or fight.